FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20151052
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA28900.081.964981
TTACGCG11950.081.282279
CGCCGAA30350.077.0794751
TTTGCGC31650.076.7026751
GTGCGAC33850.074.760141
CGGTACG28600.072.793181
GCGCTAG33750.071.276161
TTACCCG40450.068.199791
GCGCGTA35400.068.161781
ACGACCG29500.067.3305361
TAGGCGC34850.067.12661
GGCGACT37750.064.503211
TCACGCG26800.064.1021358
GCCGGAT40300.063.707521
CGAACGC39250.063.5375371
ATAACGC38800.063.3264351
GACGCGT32700.062.766341
GTTCGCG41900.062.6258248
CCGCTAT37950.062.4186551
AACCGCG36500.062.278181
Produced by FastQC (version 0.11.5)