FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20150020
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGC31950.081.2029341
TGCGCGA30500.080.4849241
CGCCGAA31550.080.135871
TTACGCG12900.076.984159
GTGCGAC34150.076.6173551
GCGCTAG34850.072.7585751
CGGTACG34200.072.207291
GGCGACT39350.068.7338941
ATAACGC35950.067.8745651
CCGCTAT41850.067.789081
GTTCGCG41250.067.480418
TTACCCG37550.066.352551
GACGCGT32250.066.3179551
TAGGCGC36100.064.538651
TCACGCG27600.064.346888
ACGACCG29000.064.119541
CGAACGC36500.064.0327451
AACGCGT28100.063.721173
CCTTACG37150.062.9123881
GCGCGTA33450.061.9613461
Produced by FastQC (version 0.11.5)