FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20009161
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA31550.082.261621
TTACGCG10300.078.825159
CGCCGAA28850.075.688951
TTTGCGC31100.075.414031
CGGTACG32400.074.203541
GTGCGAC36050.070.973441
TTACCCG37750.069.919671
CGAACGC38600.068.5704651
ACGACCG31300.068.120141
GCGCTAG34850.067.721121
GCGCGTA35350.067.595191
ATAACGC37350.067.5189061
GACGCGT30700.065.859161
GTACCGT41550.064.5527348
GGCGACT38450.064.439991
GTTCGCG41500.064.106488
GCCGGAT39800.062.8083951
CCGCTAT40800.062.7105981
GTTACGC27800.062.582148
TCACGCG30800.062.3704838
Produced by FastQC (version 0.11.5)