FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18915236
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC27150.0100.1200565
CGACGCT27700.098.3945856
CACGACG30000.091.332324
GACGCTC41250.066.2491767
TACACGA44300.062.1874432
CTACACG48550.058.0295261
ACGCTCT50300.054.3295948
ACACGAC53100.051.736693
CGCTCTT62450.043.875549
CTTCCGA84700.07.61674210-14
TCTTCCG84850.07.55201910-14
TTCCGAT97800.06.922622710-14
TCCGATC96900.06.852231515-19
CGCTAGT11100.0095740316.6269921
CCGATCT104150.06.51443815-19
CGCTGAG37950.06.39648871
CGATCTA28500.06.20590815-19
CGCTAAT17506.022541E-45.88476941
TAACCCT742800.05.8356936
CTAACCC787400.05.62477875
Produced by FastQC (version 0.11.5)