FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16971925
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA28100.081.99731
TTACGCG11850.081.352999
TTTGCGC27500.079.516971
CGGTACG27750.074.0408251
CGCCGAA28750.074.017831
ACGACCG24400.073.079011
CGAACGC29850.070.30691
GCGCTAG28400.069.504061
GTGCGAC28850.069.437331
CCGCTAT33600.067.046371
TTACCCG33000.066.931221
GCGCGTA27050.065.377151
TAGGCGC33850.064.600191
GGCGACT35600.064.5164641
GTTACGC26700.064.339458
TCCGCGA25750.063.898248
GCCGGAT35800.063.5411221
CGAACTA35350.063.312091
CGATTCG20600.062.6330074
GACGCGT26150.062.2956161
Produced by FastQC (version 0.11.5)