FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18002537
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACCG26200.082.688631
CGCCGAA29050.078.115531
GTGCGAC31950.076.3119051
TGCGCGA26250.074.6976551
TTTGCGC30300.073.681081
CGGTACG29300.073.438711
TTACGCG10100.072.4896559
GCGCTAG30250.069.190191
GGCGACT37750.066.921631
ATAACGC33000.066.5399251
TTACCCG34700.066.031351
GACGCGT28500.065.4506451
CCGAACG18550.064.087889
ACGCCGA33950.063.8127251
GCGCGTA28600.063.4243431
CCGCTAT34650.062.735521
CGAACGC33550.062.3845981
TTCCGCG15350.062.3363767
GTTCGCG36200.062.277028
CCTTACG34250.061.7528421
Produced by FastQC (version 0.11.5)