FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18002537
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC25150.096.548035
CGACGCT25150.095.972696
CACGACG27250.090.969844
GACGCTC37250.065.575847
TACACGA41750.060.600912
CTACACG42100.060.5152321
ACGCTCT46550.053.0976038
ACACGAC47650.053.0887873
CGCTCTT60250.040.9036529
CTTCCGA76000.07.725431410-14
TCTTCCG77300.07.257930810-14
CGAAAAG32800.06.93913271
TTCCGAT89750.06.88107810-14
TCCGATC89050.06.756249415-19
CCGATCT99300.06.394643315-19
AACGGGG32408.6583896E-106.1183751
TAACCCT489650.05.9804946
CGTTGAG24801.6294689E-65.92100761
CTAACCC507700.05.86774545
AACCCTA534400.05.6054571
Produced by FastQC (version 0.11.5)