FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16799426
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA27100.083.755471
TTACGCG11100.079.0213249
CGGTACG27100.077.250191
ACGACCG24900.076.700461
TTTGCGC26650.073.317631
GTGCGAC28200.071.3716051
GACGCGT24650.070.92251
CGCCGAA28250.070.725241
GGCGACT34450.068.871151
GCGCGTA27300.066.459691
ATAACGC29500.066.2344061
TTACCCG31850.065.729361
CGAACGC31150.065.0841141
ACGCCGA32800.064.721411
CGAACTA35400.064.532891
GTTACGC25150.063.987768
TTCCGCG17300.063.6911937
GCGCTAG27450.063.6881451
CGTTGCG14500.063.4918027
GCCGGAT31800.063.0608061
Produced by FastQC (version 0.11.5)