FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16799426
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC26100.099.919965
CGACGCT26700.096.58936
CACGACG28150.092.386034
GACGCTC36200.071.041177
TACACGA39650.066.7231452
CTACACG44400.059.9165421
ACACGAC46150.057.2939573
ACGCTCT46550.055.247078
CGCTCTT57800.044.61939
CTTCCGA77200.08.31414910-14
TCTTCCG77500.07.94545210-14
TTCCGAT83050.07.79828810-14
TCCGATC80450.07.582050315-19
CCGATCT90350.06.943690315-19
CGATCTA22800.06.60889815-19
AACGGAG35051.4006218E-106.08038571
TAACCCT563700.05.4745936
CGTAGAG25751.8880959E-55.42247441
CGTTGAG20507.721215E-45.3772291
CTAACCC591900.05.2994385
Produced by FastQC (version 0.11.5)