FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17936769
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACCG27400.078.644971
TTACGCG10600.077.961319
TGCGCGA26900.077.919571
CGCCGAA27700.077.793231
GTGCGAC32850.075.6720051
CGGTACG27300.073.006351
TTTGCGC29200.072.033931
GCGCGTA26950.068.496581
TAGGCGC34050.068.253341
CCTTACG34100.066.643541
GCCGGAT34250.066.566411
TTCCGCG14450.066.2206657
CGAACGC33550.066.201581
CGCGGTA35500.066.087031
CCGCTAT34300.065.826111
GGCGACT35150.065.071231
AACGCGT26650.064.467163
GCGCTAG29800.064.413691
GACGCGT26600.064.144631
GTACGCT27100.063.93068
Produced by FastQC (version 0.11.5)