FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17936769
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC25300.096.8372045
CGACGCT25350.096.360266
CACGACG27350.089.8438644
GACGCTC37850.064.537197
TACACGA40950.061.782922
ACACGAC44450.056.9114573
CTACACG44700.056.107051
ACGCTCT44200.054.9379168
CGCTCTT58050.041.8304259
CTTCCGA73550.07.844745610-14
TCTTCCG74500.07.49174410-14
TTCCGAT86100.06.835988510-14
TCCGATC82650.06.82314315-19
CCGATCT90650.06.380913315-19
CGAAAAG31151.299486E-85.9200861
TAACCCT483350.05.68360046
ACCGGGT23402.2480343E-55.6741751
CGTTGAG23752.8503993E-55.59055571
CTAACCC500900.05.51340635
CCTAACC520000.05.49210644
Produced by FastQC (version 0.11.5)