FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20089191
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG1920583795.60283935774217No Hit
NAGTAGAG2953161.470024352897038No Hit
AAGTCGAG657420.32725060954420715No Hit
AAGGAGAG624930.31107773329448657No Hit
AAGTGGAG480340.23910370507204595No Hit
AAGTTGAG421270.2096998331092576No Hit
AAGTAGCG415190.20667332995141516No Hit
ACGTAGAG326100.16232609864677974RNA PCR Primer, Index 17 (100% over 8bp)
AAGCAGAG318510.15854794750072315No Hit
AAGTAGGG315870.15723380797165998No Hit
AAGAAGAG310760.15469015153472332No Hit
CAGTAGAG243080.12100039269874034No Hit
AAGTAGAA223490.11124888005694207No Hit
AGGTAGAG223420.11121403544821691No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)