FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20384926
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12500.086.98179
CGCCGAA31350.079.723651
TTTGCGC32600.076.8949361
TGCGCGA30850.074.987811
CGGTACG30700.074.8696061
ACGACCG29900.073.638681
GACGCGT32500.071.867361
GTGCGAC36250.070.383611
TTACCCG37500.068.8309251
GCGCTAG34000.068.696751
ATAACGC39600.067.998421
TAGGCGC39350.065.594921
CCGCTAT39300.065.48911
TCGTTCG40850.065.120938
CGAACGC38000.064.989011
AACGCGT28200.064.291223
TCGTACG39050.063.8533978
TCACGCG29200.063.05188
TCGCGAT24500.063.0173538
GGCGACT40250.062.834541
Produced by FastQC (version 0.11.5)