FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16764463
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC26450.0107.698525
CGACGCT27000.0104.967436
CACGACG29300.097.470114
GACGCTC37500.075.5765467
TACACGA40850.070.627952
CTACACG45000.064.432391
ACACGAC47750.061.0232773
ACGCTCT46650.060.753218
CGCTCTT59050.048.2410559
CTTCCGA72150.09.18239710-14
TCTTCCG72650.09.09924610-14
TCCGATC79300.08.26312915-19
TTCCGAT80250.08.12912310-14
CCGATCT86750.07.75403615-19
CGATCTA25350.07.14844215-19
CGCTGAG31753.1141099E-96.04162551
CGCAAAG26558.1085636E-75.83551171
GTTCCGA23052.3374254E-55.66036465
AACGGGG29901.2573018E-65.42844771
TAACCCT580000.05.3988016
Produced by FastQC (version 0.11.5)