FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18067097
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACCG27650.079.2139361
TTACGCG11200.078.959169
TGCGCGA28650.078.757911
CGCCGAA28450.077.244821
TTTGCGC29650.077.093211
GCGCTAG31100.071.608191
GTGCGAC33900.071.113921
CGGTACG28000.070.611381
CGCGGTA35500.069.5650941
ATAACGC34400.069.43931
TCGTACG34100.068.8733758
GCGCGTA27350.066.914841
ACGCCGA36350.066.523021
CGAACGC32950.066.472391
GCCGGAT36250.065.692761
AACGCGT26050.065.674243
CCGCTAT36850.065.62041
GACGCGT29400.065.498961
GGCGACT37800.064.943411
AACCGCG33300.064.890861
Produced by FastQC (version 0.11.5)