FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18067097
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT25100.0100.7865456
ACGACGC25450.0100.254925
CACGACG26900.094.04224
GACGCTC37300.067.822267
TACACGA41250.062.04562
CTACACG42600.060.7625731
ACACGAC44800.057.4380873
ACGCTCT45300.056.6444178
CGCTCTT59850.043.600399
CTTCCGA77500.07.89388210-14
TCTTCCG77350.07.53434710-14
TTCCGAT84100.07.481240310-14
TCCGATC80950.07.05616715-19
CCGATCT90450.06.523419415-19
ACGGGAG39801.8189894E-126.09724951
CGATCTA26750.05.96153915-19
CGCTGAG34757.057679E-105.9252451
AACGGGG32405.329639E-95.9010791
CGTTGGG28304.6469358E-75.71662041
ACCGGAG24709.2955215E-65.65665531
Produced by FastQC (version 0.11.5)