FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16842745
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA26100.082.431891
TTTGCGC27750.079.3828051
TTACGCG10700.077.910069
ACGACCG22600.077.6528851
GTGCGAC27050.075.1935651
CGCCGAA28850.075.083471
CGGTACG25900.074.846641
TTACCCG35150.073.115741
GCGCTAG28900.072.158681
GCGCGTA29650.067.85691
GGCGACT33400.066.393971
CGAACGC29650.066.370991
GACGCGT25400.065.0454561
CGTTGCG12100.064.702037
TCCGCGA24450.064.6334158
GTACCGT39700.063.360488
GTACGCT24600.062.765938
ATAACGC30350.062.6627161
CCGCTAT32500.062.584181
GCCGGAT32750.062.1064341
Produced by FastQC (version 0.11.5)