FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16842745
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT26950.0107.85296
ACGACGC28550.0102.061295
CACGACG30000.096.886424
GACGCTC37600.078.4610447
TACACGA42800.068.429472
ACGCTCT45350.064.413268
CTACACG45750.064.029331
ACACGAC46250.063.002533
CGCTCTT58050.050.3212749
CTTCCGA78850.08.56778210-14
TCTTCCG75450.08.531157510-14
TTCCGAT88200.07.725267410-14
TCCGATC86350.07.722984315-19
CCGATCT95200.07.14209315-19
CGGGTTA25056.2409526E-96.74089051
CGTAGAG26652.2400855E-86.3361841
CGTAAAG22552.0383886E-66.1859191
CGATCTA29150.06.017781715-19
TCGCCGG17205.7210773E-45.9084234145
TAACCCT568550.05.8518116
Produced by FastQC (version 0.11.5)