FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18567586
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12300.088.985129
TTTGCGC30200.078.590621
TGCGCGA27800.077.346391
ACGACCG26100.076.682941
CGGTACG28050.073.739281
TTACCCG37450.072.117691
CGCCGAA27000.071.9223251
GCGCTAG31550.071.454611
GTGCGAC32750.069.9723361
ATAACGC34050.067.519371
GTTACGC29000.066.4858558
CCGCTAT35100.064.863631
TCGTACG35050.064.315848
GTACCGT39450.064.1245048
CGACACG36800.063.889021
GCCGGAT38050.063.7455561
TAGGCGC33550.063.646771
GGCGACT35600.062.9076541
GACGCGT29750.062.523031
ACGCCGA33000.062.452931
Produced by FastQC (version 0.11.5)