FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18567586
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT24650.089.100656
ACGACGC25600.086.359785
CACGACG27350.082.158064
GACGCTC35950.061.2963377
TACACGA41250.055.1843382
CTACACG44650.051.076721
ACGCTCT44250.050.2902458
ACACGAC52750.043.5597043
CGCTCTT58300.038.1705559
CTTCCGA68950.07.695442710-14
TCTTCCG71900.07.278888710-14
TCCGATC76950.06.989652615-19
TTCCGAT78600.06.87975710-14
CCGATCT86800.06.43030115-19
TAACCCT675650.05.91132646
ACGGGAG43153.6379788E-125.79670141
CTAACCC716450.05.7668765
ACCCTAA750300.05.63305142
CCTAACC744200.05.6004644
CCCTAAC753600.05.5787283
Produced by FastQC (version 0.11.5)