FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19175809
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12000.080.9475569
TGCGCGA28700.080.872691
GTGCGAC34100.078.189591
CGCCGAA31250.076.858951
ACGACCG29500.076.438671
TTTGCGC27700.075.572151
CGGTACG30950.073.332161
CGTTGCG14500.067.991137
CCGCTAT35450.067.3385541
TTACCCG37300.066.361741
GACGCGT31050.066.2358551
GCGCTAG32550.065.440061
ACGCCGA36400.065.177521
TCGGCGC24550.064.960978
ACGCTAA46200.064.388711
GTTACGC28450.063.9547428
GCGCGTA32050.063.9400331
CGAACTA41300.063.669241
TAGGCGC36650.063.5304571
GGCGACT37200.063.3809551
Produced by FastQC (version 0.11.5)