FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21493545
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG13950.085.221339
TGCGCGA32300.080.92331
TTTGCGC34700.078.2885741
CGGTACG34300.076.846921
ACGACCG30350.074.026781
CGCCGAA35350.069.787241
GCGCTAG38000.068.7848051
GTGCGAC35750.068.184891
AACCGCG39850.067.618241
ATAACGC39500.066.730361
ACGCCGA43000.066.421291
GTTACGC33050.066.019728
GGCGACT42050.065.128181
TAGGCGC41850.065.088551
TCGTACG42550.064.9088758
TTACCCG37900.064.898061
CGAACTA45300.064.021381
GCCGGAT44050.063.6712761
CGCGGTA38950.063.1485671
CGCGTGA38850.062.933131
Produced by FastQC (version 0.11.5)