FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21559870
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGC34550.081.267011
TTACGCG13150.079.378389
TGCGCGA31500.076.5353851
CGCCGAA34800.076.247731
GCGCTAG36450.073.60281
ACGACCG33650.072.955891
GTGCGAC36350.070.3677751
ATAACGC40850.067.2943341
CCTTACG39900.066.501781
CGGTACG33300.065.555811
GGCGACT40700.064.4722441
CTTACCG46300.064.453131
TTACCCG43900.063.9584351
GCGCGTA35750.063.5303731
TAGGCGC40650.063.105011
GACGCGT34300.063.0016821
GTACGCT33400.062.9385958
GTTACGC32550.062.5778738
ACGCCGA42950.062.2927061
CGAACGC41900.061.748671
Produced by FastQC (version 0.11.5)