FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19269471
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12550.080.861679
ACGACCG26550.078.161571
TGCGCGA27400.076.005421
CGCCGAA30650.075.388981
TTTGCGC30850.074.184621
GTGCGAC34100.072.940881
CCGCTAT37300.069.642531
GCGCTAG34100.069.056461
GCGCGTA31300.068.180841
CGGTACG31450.067.8556441
ATAACGC34150.065.5075761
GACGCGT30550.063.5918431
TTACCCG36850.063.5035931
CCTTACG34600.062.528771
TCGTACG37100.062.5222328
CGAACTA40000.062.366071
CGTTGCG15350.062.3336877
GTTACGC28800.062.1674278
CGCGGTA40050.062.104471
CGAACGC32350.061.6458241
Produced by FastQC (version 0.11.5)