FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19253791
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC31350.098.036285
CGACGCT31200.097.345966
CACGACG34650.088.071674
GACGCTC42400.071.461347
TACACGA45650.067.5034942
CTACACG51550.060.214451
ACGCTCT50550.059.9395688
ACACGAC52950.057.9070933
CGCTCTT64900.047.46829
CTTCCGA85650.08.37853610-14
TCTTCCG85600.08.1124510-14
TTCCGAT94050.07.59938510-14
TCCGATC97300.07.15195515-19
CGATCTA28700.06.768918515-19
CGCTGAG38500.06.6868911
CCGATCT108600.06.674773715-19
CGCGACC14400.00311371756.0469055145
TAACCCT747250.06.043396
AACGGGG31903.7143764E-95.9951441
CGAAAAG33201.4770194E-95.9819481
Produced by FastQC (version 0.11.5)