FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19196208
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACG30700.082.058941
TGCGCGA29950.077.7378541
GTGCGAC33600.076.72521
TTTGCGC31800.075.986931
CGCCGAA28850.075.610271
ACGACCG25550.073.0151441
TTACGCG11700.071.8700569
GACGCGT31350.069.580741
GCGCTAG35400.069.296821
TTACCCG38250.067.781851
TAGGCGC33950.066.631731
CGCGGTA36950.066.588691
GCGCGTA31550.065.647761
CGAACGC33500.065.115111
AACGCGT27700.064.4158253
CCTTACG36550.064.3032151
GGCGACT36900.063.8923381
ACGCCGA38200.063.8329471
CGAACGT21050.062.0236933
GCCGGAT36900.060.9067151
Produced by FastQC (version 0.11.5)