FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19196208
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT29050.0100.056016
ACGACGC31700.092.376725
CACGACG33200.089.076834
GACGCTC39200.074.148657
TACACGA46200.065.1205142
CTACACG49850.061.125361
ACGCTCT48700.060.4285288
ACACGAC53300.056.5732423
CGCTCTT64500.045.850649
CTTCCGA86450.08.1835210-14
TCTTCCG84150.07.90758610-14
TCCGATC98500.07.079599415-19
TTCCGAT101200.07.048065710-14
CCGATCT110300.06.729677715-19
AACGGGG34004.292815E-106.04668861
CGATCTA30350.05.8277515-19
TAACCCT778200.05.78422646
CTAACCC827650.05.71006775
ACCCTAA841300.05.66143042
CCCTAAC857550.05.65472653
Produced by FastQC (version 0.11.5)