FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005486150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19949760
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG1901857695.33235487544712No Hit
NAGTAGAG3728601.8689949152270502No Hit
AAGGAGAG611090.3063144619283641No Hit
AAGTCGAG549160.27527148196269025No Hit
AAGTGGAG379660.19030805383122404No Hit
AAGTTGAG364670.1827941789775917No Hit
AAGTAGCG350540.17571138700415442No Hit
AAGTAGAA323820.16231774216832684No Hit
AAGCAGAG319240.1600219752017067No Hit
AAGAAGAG313740.15726504980511044No Hit
ACGTAGAG309740.15526001315304044RNA PCR Primer, Index 17 (100% over 8bp)
AGGTAGAG258750.1297008084307781No Hit
ANGTAGAG231050.11581592961519337No Hit
AAGTAGGG207930.10422681776622877No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)