FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23103096
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCCAGA2212676395.77401660799055No Hit
NGTCCAGA4653882.0143966851888595No Hit
GGGCCAGA561580.2430756466579198No Hit
GGCCCAGA411070.17792853390731703No Hit
GGTCCAGC407100.17621014949684666No Hit
GGACCAGA326520.14133170723092697No Hit
GGTCCCGA316540.13701193987160853No Hit
GGTTCAGA250550.10844866852477261Illumina DpnII expression Adapter 1 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)