FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005486152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20365301
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG1934019894.96642352597686No Hit
NAGTAGAG4149742.0376521810308623No Hit
AAGGAGAG646410.31740753549382844No Hit
AAGTCGAG529010.2597604621704339No Hit
AAGTGGAG449730.22083150158202913No Hit
AAGTTGAG399770.19629957838580436No Hit
AAGTAGCG343870.16885092933318294No Hit
AAGAAGAG339950.16692608668047676No Hit
AAGCAGAG325770.1599632630030855No Hit
ANGTAGAG305070.1497989153217033No Hit
ACGTAGAG302150.1483651039579528RNA PCR Primer, Index 17 (100% over 8bp)
AAGTAGAA298080.14636660661190326No Hit
AAGTAGGG294030.14437792989163284No Hit
CAGTAGAG238270.1169980252194652No Hit
AGGTAGAG238190.11695874271634875No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)