FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16719486
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACCG26100.083.333591
TGCGCGA24450.078.938251
CGCCGAA28050.076.7464141
TTACGCG10250.076.375829
TTTGCGC27750.074.901071
CGGTACG24400.071.494241
GCGCTAG26750.069.098491
GTGCGAC27400.068.272061
GCGCGTA25700.068.166631
CGTTGCG14250.067.145227
CCGCTAT31900.066.087691
TTACCCG34700.065.88921
ATAACGC28650.064.775141
GCCGGAT32400.064.3804861
CGCGGTA34100.064.000881
CGAACGC31350.063.6953471
GGCGACT32450.062.9087371
CGAACTA35300.062.8766561
TCGTACG30450.062.8452728
GTACCGT36400.062.728548
Produced by FastQC (version 0.11.5)