FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20567545
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG14400.085.071119
CGGTACG30500.078.622751
CGCCGAA30550.078.2510451
TTTGCGC33200.077.371941
TGCGCGA30400.076.927651
ACGACCG30450.076.5575261
GACGCGT31500.071.648741
GTGCGAC34050.070.643691
TTACCCG39200.070.074691
CCTTACG39250.069.985421
CCGCTAT40050.069.143571
CGAACGC37750.068.6363141
GTTACGC34450.067.752388
ATAACGC37800.067.563511
GCGCTAG34900.067.008611
TAGGCGC38700.066.18411
GGCGACT39550.064.9493941
AACGCGT28950.063.097633
CGATCGG37750.061.7530171
TCGTACG42500.061.570998
Produced by FastQC (version 0.11.5)