FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18872741
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT24350.092.580276
ACGACGC25550.089.6506045
CACGACG27000.085.10454
GACGCTC37400.060.4703257
TACACGA39600.059.1411552
CTACACG44600.053.100541
ACGCTCT44050.051.505048
ACACGAC52250.044.8098223
CGCTCTT59550.038.585919
CTTCCGA72950.07.531781710-14
TTCCGAT83150.06.869383310-14
TCTTCCG74700.06.85074610-14
TCCGATC81850.06.535804715-19
CGCAAAT26852.5171175E-86.3003031
CCGATCT90650.06.221185715-19
TAACCCT625250.05.83137946
ACCCTAA683950.05.68228342
CTAACCC660250.05.65400085
CCTAACC677350.05.62897874
CCCTAAC692450.05.6109113
Produced by FastQC (version 0.11.5)