FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18841528
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT23500.088.5264746
ACGACGC24200.086.56445
CACGACG27300.076.734334
GACGCTC34750.060.2840167
TACACGA37550.056.7616842
CTACACG42750.049.9845961
ACGCTCT43700.047.605778
ACACGAC49350.042.8896563
CGCTCTT55700.037.479739
CTTCCGA66750.07.53649910-14
TCCGATC70950.07.33578215-19
TTCCGAT74150.07.01899210-14
TCTTCCG69700.06.90552610-14
CCGATCT80300.06.46356115-19
TAACCCT641250.05.83285866
ACGGGAG44251.8189894E-125.80809971
CTAACCC680750.05.71799145
AACGGGG33501.1748853E-85.6991211
AACCCTA696150.05.6432631
CGCAAAT27501.6963913E-65.60745671
Produced by FastQC (version 0.11.5)