FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20365301
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12300.084.269999
TGCGCGA34350.083.751081
CGCCGAA29850.080.8877261
TTTGCGC33200.078.4732061
ACGACCG33350.076.799911
CGGTACG33100.076.7148061
GCGCTAG35100.073.80721
GTGCGAC37700.070.469031
CGAACGC38950.070.280121
CGTTGCG15350.069.8868267
GACGCGT34350.067.299981
GTACCGT45250.065.676088
GGCGACT39100.065.31811
CCGCTAT39550.064.946041
TCACGCG26050.064.553868
ATAACGC37000.064.0666351
AACCGCG36000.064.0115361
CGCGGTA42050.063.004611
TCGTACG39050.062.924628
GTTCGCG44400.062.6888438
Produced by FastQC (version 0.11.5)