FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18120052
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC25250.098.454185
CGACGCT26200.094.0564656
CACGACG27250.092.2920764
GACGCTC39000.062.6292957
TACACGA40850.061.098352
CTACACG45050.055.872311
ACGCTCT46350.053.1661728
ACACGAC47100.053.08843
CGCTCTT59000.041.5212979
CTTCCGA76850.07.394321410-14
TCTTCCG77300.07.220002710-14
TTCCGAT86850.06.743225610-14
TCCGATC84800.06.718353715-19
CCGATCT94900.06.354674315-19
CGCAAAT25704.147114E-76.04921051
CGCTAAG16000.00122473846.0150031
AACGGAG37757.566996E-105.68713571
CTAACCC524800.05.66228155
CCTAACC540500.05.65871954
TAACCCT504950.05.64098836
Produced by FastQC (version 0.11.5)