FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19877610
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12000.080.943549
TGCGCGA31550.080.922051
ACGACCG27900.077.795521
CGGTACG33500.074.8938751
TTTGCGC32750.074.5870741
GTGCGAC33100.074.465231
CGCCGAA28850.072.6834951
CGAACGC38150.069.043891
GCGCGTA34400.068.2290041
GCGCTAG32700.067.726011
ATAACGC38900.066.388721
TTACCCG37150.066.347221
AACGCGT27000.065.775523
CCGCTAT38200.064.7161561
GTTACGC28900.063.95898
AACCGCG35850.063.622291
GACGCGT32450.063.2595751
GTTCGCG42550.063.2023168
TCGCGAT24500.062.7232328
TAAACCG43500.062.5819741
Produced by FastQC (version 0.11.5)