FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20720610
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG11600.082.4887859
TGCGCGA27800.076.585371
TTTGCGC32950.075.755841
CGCCGAA31650.073.996231
ACGACCG32100.073.1876141
GTGCGAC35550.072.4871
GACGCGT34350.072.4545441
CGGTACG32250.072.3919141
GCGCTAG34100.070.186861
ATAACGC38600.068.090781
GGCGACT40450.066.791611
GCGCGTA31500.066.657361
TCACGCG32400.066.001828
CGAACGC40100.065.543751
CCGCTAT41900.065.531521
GCCGGAT38250.064.875081
CCTTACG39600.064.517371
TTACCCG40300.063.943251
AACCGCG42400.063.892981
TAGGCGC36600.063.1861761
Produced by FastQC (version 0.11.5)