FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19877419
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG1896416895.40558560444894No Hit
NAGTAGAG3064911.5419054153861727No Hit
AAGGAGAG657390.3307220117460924No Hit
AAGTCGAG633970.3189397979687403No Hit
AAGTGGAG487300.24515255225037014No Hit
AAGTTGAG424480.21354885158883052No Hit
AAGTAGCG409770.2061484944297849No Hit
ACGTAGAG351170.17666780581523184RNA PCR Primer, Index 17 (100% over 8bp)
AGGTAGAG333320.16768776670653268No Hit
AAGAAGAG320750.161364008073684No Hit
AAGCAGAG312430.15717835399052563No Hit
AAGTAGGG277660.13968614335694185No Hit
AAGTAGAA267730.13469052496201844No Hit
CAGTAGAG224650.11301769107951087No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)