FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21439688
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG13500.078.934449
TGCGCGA32950.078.793741
TTTGCGC33150.075.655971
GTGCGAC34000.074.629851
CGGTACG31050.072.2454761
CGCCGAA35400.072.093921
GCGCTAG38450.067.3315661
ACGACCG34750.066.4581
TTACCCG39600.066.119151
ATAACGC42150.065.957871
TAGGCGC41000.064.937731
GGCGACT43050.064.578961
CGAACTA47150.064.1111
CCTTACG41200.063.7299231
CGAACGC41300.063.2194481
GACGCGT34650.062.829111
AACGCGT29900.062.551183
CCGCTAT41400.062.3561331
ACGCCGA42850.062.305761
GTACCGT49650.062.197558
Produced by FastQC (version 0.11.5)