FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20843615
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG15000.079.2602169
TGCGCGA33850.079.0793841
ACGACCG29550.075.736431
CGCCGAA34500.075.681551
TTTGCGC32900.072.6930161
GCGCTAG35050.071.781291
GTGCGAC35300.070.858561
CGGTACG31050.068.30891
CGAACGC38050.065.7373661
ATAACGC38700.065.5781861
TAGGCGC38600.065.179651
GTACCGT45050.064.850448
TTACCCG38850.064.7602161
GACGCGT34350.064.088581
AACCGCG40900.064.017781
GTTACGC34850.062.8212858
CCGATAA43600.062.2340851
GCGCGTA36350.062.1720161
CCTTACG39800.061.9280931
GTACGCT31850.061.9101878
Produced by FastQC (version 0.11.5)