FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18437007
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG1758730495.39131812446566No Hit
NAGTAGAG3080811.6709924772496967No Hit
AAGTCGAG648410.3516894038170078No Hit
AAGGAGAG629300.341324380904124No Hit
AAGTAGCG420400.22801965633575993No Hit
AAGTTGAG368280.19975042586901443No Hit
AAGTGGAG364360.19762426732278185No Hit
AAGCAGAG355730.1929434641967647No Hit
ACGTAGAG353690.19183699393290896RNA PCR Primer, Index 17 (100% over 8bp)
AAGAAGAG331280.17968209265202317No Hit
AGGTAGAG238540.12938108663732678No Hit
AAGTAGGG219690.1191570844443461No Hit
AAGTAGAA205020.11120026151750118No Hit
CAGTAGAG201170.10911207008816562No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)