FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16439935
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA26900.081.5657041
TTACGCG9800.079.890319
TTTGCGC27400.076.340341
GCGCTAG27100.076.105921
ACGACCG25450.075.579941
GTGCGAC27500.071.009621
CGGTACG26850.070.277131
CGCCGAA27150.068.423061
TTACCCG31200.067.511311
GTACCGT38600.067.4225168
GACGCGT25050.067.151961
CGAACGC30650.067.052421
CGCGGTA32050.066.633621
ATAACGC29600.064.242181
GCGCGTA27600.064.1275941
GGCGACT34850.063.7983471
TCGCGAT20750.063.2351078
TTCCGCG13300.062.6821637
AACGCGT21550.062.5696143
TCGTACG31350.061.740698
Produced by FastQC (version 0.11.5)