FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17717292
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT21250.089.0399556
ACGACGC23200.082.80595
CACGACG25050.076.691144
GACGCTC32600.058.2622267
TACACGA35050.055.4466322
CTACACG45600.044.8526231
ACGCTCT43200.043.798598
ACACGAC47950.040.669223
CGCTCTT54550.035.084289
CGCAAGT15651.8721532E-57.0515541
CGATGAG29606.6211214E-106.46233651
TCTTCCG73050.06.27176410-14
CTTCCGA76400.06.09164410-14
AACGGAG32801.1023076E-96.0561671
AACGGGG30458.349161E-96.040331
GTTCCGA27806.880691E-85.9977235
AACGAGG30756.4907E-85.74214361
TAACCCT565100.05.7087116
CGCTAAT17000.00272007615.6260341
TCCGATC85650.05.53535415-19
Produced by FastQC (version 0.11.5)