FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18967554
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA31000.082.125651
TTACGCG11100.079.678219
ACGACCG30000.076.571941
CGCCGAA28350.076.12551
CGGTACG32100.075.436961
TTTGCGC34100.074.8742141
GCGCTAG33300.073.37761
GTGCGAC35650.072.6448751
CGAACGC36500.068.547951
GGCGACT38900.068.08011
ATAACGC35350.067.6736451
GCGCGTA31650.067.494811
GACGCGT31300.066.613431
TCGTACG37300.065.1089868
GTACCGT41150.064.831048
GTCGGAC28650.063.582261
CGCGGTA37500.062.2329861
AACGCGT25700.061.4938663
GTTCGCG41150.061.4837848
GCCGGAT36550.061.2484741
Produced by FastQC (version 0.11.5)