FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18104980
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT23600.092.4604956
ACGACGC24850.088.393015
CACGACG25500.086.425594
GACGCTC33750.065.942457
TACACGA37900.058.927772
CTACACG41800.054.552931
ACGCTCT42900.052.3847588
ACACGAC46950.047.71223
CGCTCTT57950.039.7808659
CTTCCGA82550.06.410638310-14
TCTTCCG79700.06.330622710-14
CGGTGAT40100.06.23687741
TAACCCT640000.06.1166776
GTTCCGA30952.593879E-96.089965
CTAACCC685400.05.80670835
CCCTAAC716050.05.6999573
CCTAACC708700.05.68749864
AACCCTA695500.05.66891961
ACCCTAA707750.05.66570232
TCCGATC102550.05.31589315-19
Produced by FastQC (version 0.11.5)