FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18654096
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAA28750.083.151671
TGCGCGA29350.082.1990661
TTACGCG12600.077.672379
GTGCGAC32550.074.342661
TTTGCGC30550.072.269791
ACGACCG28950.070.203261
ATAACGC33600.067.667821
CGGTACG28250.067.0258561
CGAACGC33250.066.841011
CCTTACG35350.066.7996441
TCACGCG25200.066.4530268
CCGCTAT35750.065.643241
GCGCGTA31450.065.320081
TTACCCG36000.064.984321
GCGCTAG29350.064.513811
TCGTACG34500.064.299218
GACGCGT29000.063.7798651
CGCGGTA32750.063.396911
GGCGACT36500.063.2929421
GTTACGC30650.062.915048
Produced by FastQC (version 0.11.5)