FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18654096
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT27650.093.077746
ACGACGC28200.091.519485
CACGACG29500.087.7326354
GACGCTC39800.064.844947
TACACGA43500.060.0185362
CTACACG48450.054.493761
ACGCTCT48700.052.696718
ACACGAC53250.049.147633
CGCTCTT62300.041.076849
CGTTGAG24557.094059E-117.46838241
TCTTCCG87950.07.07218510-14
CTTCCGA97300.06.824719410-14
CGCCGTT14755.912954E-46.389321145
TAACCCT704450.06.32903676
CGACGCC14000.00229531786.21379145
CTAACCC747100.06.0647645
CCTAACC768200.05.89821624
TTCCGAT120000.05.872015510-14
ACCCTAA769550.05.86172872
CGATGGA30054.2720785E-85.85739851
Produced by FastQC (version 0.11.5)