FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21898640
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCCAGA2090312695.45399166340923No Hit
NGTCCAGA4211481.923169658024425No Hit
GGTCCAGC555520.253677853967187No Hit
GGGCCAGA446080.20370214771328268No Hit
GGTCCCGA434980.198633339787311No Hit
GGCCCAGA397560.1815455206350714No Hit
GGTTCAGA347380.1586308556147779Illumina DpnII expression Adapter 1 (100% over 8bp)
GGTCCAGT294990.13470699550291707No Hit
TGTCCAGA274810.12549181136362805No Hit
GGTCCAGG251780.11497517654064363No Hit
GGTGCAGA242600.11078313539105625No Hit
GGACCAGA231710.10581022383125162No Hit
GATCCAGA220400.10064551953911294No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)