FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19703799
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT28800.099.92796
ACGACGC30100.096.092775
CACGACG32700.089.339794
GACGCTC40900.069.6559147
TACACGA44950.066.179982
CTACACG50300.059.0856131
ACGCTCT51000.056.2875868
ACACGAC54350.054.151953
CGCTCTT66000.043.605689
TCTTCCG93400.07.140662710-14
CTTCCGA102800.06.88262710-14
CGTAGAG28802.4356268E-96.38580851
ACCGGAG25102.9990952E-76.15479951
TAACCCT863900.06.0668546
CTAACCC927250.05.8946615
AACCCTA932950.05.8586671
TTCCGAT124050.05.797122510-14
ACCCTAA942500.05.7736752
AACGGAG39658.731149E-115.7515761
CCCTAAC965700.05.7350713
Produced by FastQC (version 0.11.5)